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Home Antibody All anti-GSK3B antibodies

Anti-GSK3B Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA319263
  • Rabbit polyclonal GSK3 Beta phospho S9 antibody
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC419542) , 20ug Explanation
100ug 325 3-7 Days
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WB(1)
IHC(1)
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Also for GSK3B (NM_002093)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenThis affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the aa 4-12 of human GSK3 beta.
Clone Name IsotypeIgG
Species Reactivityhuman Concentration1.0 mg/mL
Guaranteed Application *WB, IHC Suggested DilutionsELISA: 1:10,000 - 1:50,000, WB: 1:500 - 1:3,000, IHC: 1:200 - 1:1,000, IF: 1:200 - 1:1,000
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note Glycogen synthase kinase-3 (GSK 3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase. Two isoforms, alpha (GSK 3A) and beta, show a high degree of amino acid homology. GSK 3B is involved in energy metabolism, neuronal cell development, and body pattern formation.  GSK 3B participates in the Wnt signaling pathway and has been implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN.  GSK 3B phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA.

Reference Data

Target NameHomo sapiens glycogen synthase kinase 3 beta (GSK3B), transcript variant 1
Alternative NameGSK3beta isoform; glycogen synthase kinase-3 beta; glycogen synthase kinase 3 beta
Database LinkNP_002084
Entrez Gene 2932 Human
Function
Related Pathway
Cell cycle
Delta-Notch Signaling Pathway
Focal Adhesion
Hedgehog signaling pathway
Melanogenesis
Senescence and Autophagy
Wnt Signaling Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot using Immunochemical's Rabbit-anti-GSK3B pS9 antibody at a 1:1,000 dilution. All lanes contain human 293T whole cell lysate showing a band at 47 kDa. Cells were serum starved for 24 h prior to extraction. Key: Lane 1 Control, Lane 2 treated with IGF-1 (100 ng/ml) for 20' , lane 3 pre-treated with 10 uM LY294002 (selective PI3K inhibitor) and treated with IGF-1 (100 ng/ml) for 20'. Molecular weight markers confirm a MW of ~ 49 kDa. Use a 1:2,000 dilution of HRP Goat-a-Rabbit IgG (611-103-122) for detection.
IHC Image
Rabbit anti-GSK3B pS9 was used at a 1:200 dilution to detect GSK3B by immunohistochemistry in human ovarian cancer tumor tissue. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Alan Yen, LifeSpanBiosciences, Seattle, WA.

 

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