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Home Antibody All anti-HDAC1 antibodies

Anti-HDAC1 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA319251
  • Rabbit polyclonal anti-HDAC-1 antibody
  • FREE positive control: HEK293T cell transient overexpression lysate (LC417624) , 20ug
100ug 325 3-7 Days
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WB(2)
IHC(2)
IF(2)
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Also for HDAC1 (NM_004964)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenAnti-HDAC-1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 466-482 of Human HDAC-1.
Clone Name IsotypeIgG
Species Reactivityhuman Concentration1.33 mg/mL
Guaranteed Application *WB, IHC, IF Suggested DilutionsELISA: 1:10,000 - 1:50,000, WB: 1:1,000 - 1:5,000, IHC: 1:200 - 1:1,000
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note HDAC-1 antibody recognizes HDAC1 (also known as HD1, histone deacetylase 1, RPD3, RPD3L1) which belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.

Reference Data

Target NameHomo sapiens histone deacetylase 1 (HDAC1)
Alternative NameGON-10; HD1; RPD3; RPD3L1
Database LinkNP_004955
Function
Related Pathway
Cell cycle
Delta-Notch Signaling Pathway
EGFR1 Signaling Pathway
TGF Beta Signaling Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western Blot of Rabbit Anti-HDAC-1 Antibody. Lane 1: LNCaP prostate cancer cells. Lane 2: none. Load: 50 µg per lane. Primary antibody: HDAC-1 antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 55kDa for HDAC-1. Other band(s): none.
WB Image
Western Blot of Rabbit Anti-HDAC-1 Antibody. Lane 1: 293 whole cell lysate. Lane 2: none. Load: 35 µg per lane. Primary antibody: HDAC-1 antibody at 1:3,500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~65 kDa corresponding to human HDAC1. Other band(s): none. .
IHC Image
Immunohistochemistry of Rabbit Anti-HDAC-1 Antibody. Tissue: human lung tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: HDAC-1 antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: HDAC-1 is nuclear. Staining: HDAC-1 as brown color indicates presence of protein, blue color shows cell nuclei.
IHC Image
Immunohistochemistry of Rabbit Anti-HDAC-1 Antibody. Tissue: human prostate cancer tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: HDAC-1 antibody at 1:500 for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: HDAC-1 is nuclear. Staining: HDAC-1 precipitated purple with blue counterstain.
IF Image
Immunofluorescence Microscopy of Rabbit Anti-HDAC-1 antibody. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: HDAC-1 antibody at 10 µg/mL for 1 h at RT. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: HDAC-1 is nuclear. Staining: HDAC-1 was used with Atto 425 (shown in red). Anti-Keratin monoclonal antibody was used with Dylight 488 (shown in green) to detect Keratin. Data was collected on a STED-CW TCS-SP5 Confocal system (Leica Microsystems).
IF Image
Immunofluorescence Microscopy of Rabbit anti-HDAC1 Antibody. Tissue: A431 cells. Fixation: methanol. Antigen retrieval: blocked with normal goat serum. Primary antibody: HDAC1 antibody at 04 µg/mL for 1 h at RT. Secondary antibody: rabbit secondary antibody at 0.2 µg/mL for 45 min at RT. Localization: HDAC1 is nuclear. Staining: HDAC1 as green fluorescent signal. A-tubulin monoclonal antibody detected with ATTO 425 (colored RED). 2-color STED image, Leica Microsystems.

 

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