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Also for RBL2 (NM_005611)
|Rb2 (p130) peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).|
|human, rat and mouse
||ELISA: 1:5,000 - 1:20,000, WB: 1:500 - 1:2,000, IHC: 1:200 - 1:1,000, IP: 1:100
|0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Homo sapiens retinoblastoma-like 2 (p130) (RBL2)|
TGF Beta Signaling Pathway
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Immunohistochemical staining of mouse tissue using anti-pRb2/p130 antiserum. The staining shows the location of pRb2/p130 in developing mouse tissue. Other detection systems should yield similar results. Sections were cut at 5-7 µm, mounted on glass and dried overnight at 37°C. All sections were deparaffinized in xylene, rehydrated through a graded alcohol series and washed in phosphate-buffered saline (PBS). PBS was used for all subsequent washes and for antiserum dilution. Tissue sections were quenched sequentially in 0.5% hydrogen peroxide and blocked with diluted 10% normal goat anti-rabbit serum. Slides were incubated at 20° C for 1 h with rabbit anti-pRb2/p130 (1:500) dilution, washed, and then reacted with diluted goat anti-rabbit biotinylated antibody for 30 min. Slides were then reacted with streptavidin-peroxidase conjugate for 30 min at 20° C. Diaminobenzidine was used as the final chromogen. Negative controls for each tissue section were prepared by substituting the primary antiserum with pre-immune serum.