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Home Antibody All anti-EGFR antibodies

Anti-EGFR Antibody

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA319217 Rabbit polyclonal anti-EGFR antibody 100ug $325 3-7 Days
LC404515 EGFR HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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WB(1)

OriGene Data

ImmunogenThis affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 1189-1199 of human EGFR protein.
Clone Name IsotypeIgG
Species Reactivityhuman, mouse, rat Concentration1.0 mg/mL
Guaranteed Application *WB Suggested DilutionsELISA: 1:8,000 - 1:32,000, WB: 1:500 - 1:5,000, IHC: User Optimized
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note EGFR is a transmembrane glycoprotein that is a member of a family of protein tyrosine kinases crucial in maintaining a normal balance in cell growth and development.  Growth factor receptors are involved not only in promoting the proliferation of normal cells but also in the aberrant growth of many types of human tumors.  For example, the epidermal growth factor receptor (EGFR) is mutated and/or overexpressed in many common solid human squamous cell carcinomas including breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. Over-expression of the EGFR gene occurs in carcinomas with and without gene amplification. EGFR and erbB-2 are particularly important in breast cancer because increased production or activation has been associated with poor prognosis.  EGFR belongs to a family of growth factor receptors, which also includes ErbB-2/HER-2/neu, ErbB-3/HER-3/neu and ErbB-4/HER-4neu. EGFR can heterodimerize with each of the members of this family.

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB using Anti-EGFR antibody shows detection of a band at ~170 kDa corresponding to human EGFR present in unstimulated (lane 1) and EGF (50 ng/ml for 15 min) stimulated (lane 2) A431 whole cell lysates (arrowhead). Primary antibody was used at a 1:1000 dilution. Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).
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