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Anti-RELA Antibody

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SKU Description Amount Price Availability*  
TA319204
  • Rabbit polyclonal NFkB p65 phospho S276 antibody
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC402895) , 20ug
100ug 325 3-7 Days
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OriGene Data

ImmunogenNFkB p65 (Rel A) peptide corresponding to a region near phospho Serine 276 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH). 
Clone Name IsotypeIgG
Species Reactivityhuman Concentration1.0 mg/mL
Guaranteed Application *WB Suggested DilutionsELISA: 1:5,000 - 1:25,000, WB: 1:1,000
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note NF?B was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells. It was subsequently found in non-B cells in an inactive cytoplasmic form consisting of NF?B bound to I?B. NF?B was originally identified as a heterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NFKB1) subunits. Other identified subunits include p52 (NFKB2), cRel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NF?B subunit p65, similar to p50/p65 heterodimers. Low levels of p52 and p50 homodimers can also exist in cells. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by I?B-a. I?B-a ?binds to the p65 subunit preventing nuclear localization and DNA binding. Activators mediate a rapid phosphorylation of I?B by I?B kinase (IKK) which results in subsequent ubiquitination and proteolytic degradation. NF?B is then transported to the nucleus, where it activates transcription of target genes through binding to NF?B target sequences within the promoter. The HTLV-I protein Tax can induce constitutive NF?B activation through phosphorylation of both I?B-a and I?B-ß. The transforming protein Tax inhibits p53 transcriptional activity through the NF?B signaling pathway, specifically via the p65 (RelA) subunit. The inhibition of p53 activity is dependent upon phosphorylation of p65 (RelA) at S536 by the upstream kinase IKKß.

Reference Data

Target NameHomo sapiens v-rel avian reticuloendotheliosis viral oncogene homolog A (RELA), transcript variant 2
Alternative NameNFKB3; p65
Database LinkNP_001138610
Function
Related Pathway
Apoptosis
Delta-Notch Signaling Pathway
MAPK signaling pathway
Toll-like receptor signaling pathway

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WB Image
Affinity purified Anti-NFKB p65 (Rel A) pS276 TA319204 lot 24040 was probed against Normal (Lane 1) and TNFalpha (Lane 2) Stimulated HeLa whole cell lysates. A band was observed between 55 and 72 kD corresponding to the expected MW of NFkB p65. The observed higher MW bands have not been characterized.

 

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