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Home Antibody All anti-BDNF antibodies

Anti-BDNF Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA319152
  • Rabbit polyclonal anti-BDNF antibody
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400640) , 20ug
100ug 325 3-7 Days
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WB(1)
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Also for BDNF (NM_001143805)
cDNA Clone shRNA/siRNA Lysate Protein Request Antibody

OriGene Data

ImmunogenThis IgG fraction antibody was prepared from rabbit antiserum after repeated immunizations with recombinant truncated human BDNF protein produced in E.coli.
Clone Name IsotypeIgG
Species ReactivityHuman Concentration1.0 mg/mL
Guaranteed Application *WB Suggested DilutionsELISA: 1:10,000, WB: 1:1000
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note BDNF is a member of the nerve growth factor family of trophic factors. In the brain BDNF has a trophic action on retinal, cholinergic, and dopaminergic neurons, and in the peripheral nervous system it acts on both motor and sensory neurons. Some protein domains of BDNF are identical with those of NGF and another neurotrophic factor, designated NT-3 (neurotrophin-3). It exists as monomers and homodimers, and binds to NTRK2/TRKB. Polyclonal antibodies raised against murine NGF have been shown to cross-react with both NT-3 and BDNF. The propeptide is N-glycosylated and glycosulfated. BDNF is converted into mature BDNF by plasmin (PLG). It is expressed in brain, and highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum; it is also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.

Reference Data

Target NameHomo sapiens brain-derived neurotrophic factor (BDNF), transcript variant 7
Alternative NameANON2; BULN2
Database LinkNP_001137277
Function
Related Pathway
MAPK signaling pathway

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WB Image
Anti-human BDNF antibody in western blot shows detection of recombinant human BDNF raised in E.coli. Recombinant truncated (0.1 µg, 27 kDa) protein was loaded onto and resolved by SDS-PAGE, then transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Rockland's, Inc. Anti-Human BDNF. After washing, membrane was probed with secondary antibody Dylight™ 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody (611-143-122) diluted 1:20,000 in blocking buffer (p/n MB-070) for 30 min. at RT. Data was collected using Bio-Rad VersaDoc® 4000 MP imaging system.

 

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