Home Antibody All anti-HSP90AA1 antibodies
Also for HSP90AA1 (NM_001017963)
|This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 289-300 of human Hsp90 protein.|
|human, mouse, rat, monkey, chicken, Drosophila
||ELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:2,500, IHC: User Optimized
|0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Hsp90 is a member of the heat shock protein 90 family that functions as a molecular chaperone and has ATPase activity. Hsp90 family proteins are highly conserved between isoforms and species. Several signal transduction pathways depend on Hsp90 function including erbB2, steroid hormone receptors (such as androgen, progesterone, glucocorticoid, and aryl-hydrocarbon), and hypoxia sensing (Hif1alpha). Recent reports show that tumor cells are more sensitive to Hsp90 inhibition and that Hsp90 from tumor cells is more sensitive to small molecule inhibitors (eg 17AAG). The mechanism of this differential sensitivity of normal versus tumor Hsp90 is not known (although mutation has been ruled out). One possible mechanism may be differences in post-translational modification of tumor Hsp90.
|Homo sapiens heat shock protein 90kDa alpha (cytosolic), class A member 1 (HSP90AA1), transcript variant 1|
|EL52; HSP86; Hsp89; HSP89A; Hsp90; HSP90A; HSP90N; HSPC1; HSPCA; HSPCAL1; HSPCAL4; HSPN; LAP2|
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Western blot using Affinity Purified anti-Hsp90 antibody shows detection of a band at ~90 kDa corresponding to Hsp90 in various lysate preparations (arrowhead). Cos7 transfected with mutant K294R Hsp90 (left two lanes), Cos7 transfected with wild type Hsp90 (middle two lanes) and empty vector Cos7 cells (right two lanes) were either treated with Trichostatin A (an HDAC inhibitor) or mock treated with DMSO only, as indicated. Transfected cells express either mutant or wt Hsp90 coupled to FlagTM tag. Anti-FlagTM immunoprecipitation was performed prior to western blotting with anti-Hsp90. Personal Communication, Brad Scroggins, Urologic Oncology Branch, CCR, NCI, Rockville, MD.