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Home Antibody All anti-HSP90AA1 antibodies

Anti-HSP90AA1 Antibody


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA319143 Rabbit polyclonal HSP 90 K294 antibody 100ug $356 3-7 Days
LC400399 HSP90AA1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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Also for HSP90AA1 (NM_001017963)
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OriGene Data

ImmunogenThis affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding K294 of human Hsp90.
Clone Name IsotypeIgG
Species Reactivityhuman, mouse, rat Concentration1.0 mg/mL
Guaranteed Application *WB, IHC Suggested DilutionsELISA: 1:2,500 - 1:10,000, WB: 1:500 - 1:2,500, IHC: 5-10 ug/ml
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. This antibody reacts strongly with the acetylated form of the immunizing peptide and shows minimal reactivity with the non-acetylated form when tested by ELISA. To date, western blotting shows equivalent staining of lysates either treated or untreated with Trichostatin A (an HDAC inhibitor). Therefore, western blotting results are not definitive for demonstrating the specificity of this reagent. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 90 kDa in size corresponding to Hsp90 protein by western blotting in the appropriate cell lysate or extract.

Reference Data

Target Nameheat shock protein 90kDa alpha family class A member 1
Alternative NameEL52|HEL-S-65p|HSP86|HSP89A|HSP90A|HSP90N|HSPC1|HSPCA|HSPCAL1|HSPCAL4|HSPN|Hsp89|Hsp90|LAP-2|LAP2
Database LinkNP_001017963
Entrez Gene 3320 Human
Entrez Gene 15519 Mouse
Entrez Gene 299331 Rat
Related PathwayDruggable Genome Antigen processing and presentationNOD-like receptor signaling pathwayProgesterone-mediated oocyte maturationPathways in cancerProstate cancer

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
WB using Anti-Hsp90 acetyl K294 antibody shows detection of a band at ~90 kDa corresponding to Hsp90 in an SkBr3 cell lysate (arrowhead) after treatment with Trichostatin A (an HDAC inhibitor). WBting results do not definitively demonstrate the acetyl K294 specificity of this reagent because similar staining is seen in the control lane (no treatment with TSA). IP with anti-Hsp90 was performed prior to WBting with anti-Hsp90 acetyl K294.

IHC Image
Anti-Hsp90 acetyl K294 antibody was used at 20 µg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate nuclear and granular cytoplasmic positive staining in human prostate carcinoma at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.


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