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Anti-HIST1H3A PHOSPHO Antibody
Also for HIST1H3A (NM_003529)
|The antiserum was produced against synthesized phosphopeptide derived from human Histone H3.1 around the phosphorylation site of serine 10 (R-K-SP-T-G).|
||WB: 1:500~1:3000, IHC: 1:50~1:100, ELISA: 1:10000
|Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
|Histone H3.1 (phospho-Ser10) antibody detects endogenous levels of Histone H3.1 only when phosphorylated at serine 10.
|Homo sapiens histone cluster 1, H3a (HIST1H3A)|
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Western blot - Histone H3 (phospho S10) antibody. All lanes : Anti-Histone H3 (phospho S10) antibody. Lane 1 : EGF + Calyculin treated HeLa cells + phospho-peptide. Lane 2 : EGF + Calyculin treated HeLa cells. No phosphopeptide. Lysates/proteins at 30 µg per lane. Predicted band size : 15 kDa
Immunohistochemistry (Paraffin-embedded sections) - Histone H3 (phospho S10) antibody . staining human breast carcinoma by IHC-P (left hand panel). The right hand panel shows staining in the presence of immunizing phosphopeptide.