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Also for SMAD3 (NM_005902)
|The antiserum was produced against synthesized non-phosphopeptide derived from human Smad3 around the phosphorylation site of serine 425 (C-S-S-V-SP).|
|Human, Mouse, Rat
||WB: 1:500~1:3000, IHC: 1:50~1:100, ELISA: 1:20000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
|Smad3 (Ab-425) antibody detects endogenous levels of total Smad3 protein.
|Homo sapiens SMAD family member 3 (SMAD3), transcript variant 1|
|HSPC193; HsT17436; JV15-2; LDS1C; LDS3; MADH3|
Delta-Notch Signaling Pathway
EGFR1 Signaling Pathway
TGF Beta Signaling Pathway
Wnt Signaling Pathway
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Western blot analysis of extracts from 293 cells, treated with UV (5mins), Smad3 (Ab-425) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Smad3 (Ab-425) antibody.The picture on the right is treated with the synthesized peptide.