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Also for MAP3K8 (NM_005204)
|The antiserum was produced against synthesized non-phosphopeptide derived from human COT around the phosphorylation site of threonine 290 (R-G-TP-E-I).|
|Human, Mouse, Rat
|WB, IHC, IF
||WB: 1:300~1000, IHC: 1:50~100, IF: 1:100~1:500, ELISA: 1:40000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
|COT (Ab-290) antibody detects endogenous levels of total COT protein.
|Homo sapiens mitogen-activated protein kinase kinase kinase 8 (MAP3K8), transcript variant 1|
|c-COT; COT; EST; ESTF; MEKK8; Tpl-2; TPL2|
MAPK signaling pathway
Toll-like receptor signaling pathway
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Western blot analysis of extracts from 293 cells treated with LPS (100ng/ml, 30min), using COT (Ab-290) antibody (#TA312191, Line 1 and 2).The lane on the right is treated with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human brain tissue using COT (Ab-290) antibody (#TA312191).The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HuvEc cells, using COT (Ab-290) antibody (#TA312191).The picture on the right is treated with the synthesized peptide.