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Anti-SHC1 PHOSPHO Antibody
Also for SHC1 (NM_183001)
|The antiserum was produced against synthesized phosphopeptide derived from human Shc around the phosphorylation site of tyrosine 427 (P-S-YP-V-N).|
||WB: 1:500~1000, IHC: 1:50~100, ELISA: 1:10000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
|Shc (phospho-Tyr427) antibody detects endogenous levels of Shc only when phosphorylated at serine 24.
|Homo sapiens SHC (Src homology 2 domain containing) transforming protein 1 (SHC1), transcript variant 1|
EGFR1 Signaling Pathway
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Western blot analysis of extracts from Hela cells treated with Calyculin A (50ng/ml, 15mins), using Shc (phospho-Tyr427) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human brain tissue using Shc (phospho-Tyr427) antibodyThe picture on the right is treated with the synthesized peptide.