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Anti-PRKCA Antibody

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SKU Description Amount Price Availability*  
TA312039 Rabbit polyclonal PKC-pan (Ab-497) antibody 100ul $325 3 Weeks
LC400966 PRKCA HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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WB(1)
IHC(1)
IF(1)

OriGene Data

ImmunogenThe antiserum was produced against synthesized non-phosphopeptide derived from human PKC pan activation site around the phosphorylation site of threonine 497 (T-S-TP-F-C).
Clone Name IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration1mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:500~1:3000, IHC: 1:50~1:100, IF: 1:100~1:500, ELISA: 1:5000
BufferPhosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Note PKC-pan (Ab-497) antibody detects endogenous levels of total PKC pan activation site protein.

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot analysis of extracts from NIH/3T3 cells, treated with PMA (250ng/ml, 15mins), using PKC-pan (Ab-497) antibody.The lane on the right is treated with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using PKC-pan (Ab-497) antibody.The picture on the right is treated with the synthesized peptide.
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Immunofluorescence analysis of HuvEc cells, using PKC-pan (Ab-497) antibody.The picture on the right is treated with the synthesized peptide.
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