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[anti-HA]
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[Akt3]
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[LA]
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[JAK2]
Homo sapiens dual adaptor of phosphotyrosine and 3-phosphoinositides (DAPP1)
Synonyms:
BAM32
Immunogen
Peptide with sequence C-KLSQIRKQLNQGE, from the internal region (near C Terminus) of the protein sequence according to NP_055210.2.
Buffer
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20C. Minimize freezing and thawing.
Clone Name
Isotype
Species Reactivity
Human
Concentration
0.5ug/ul
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. (Protein A or G Sepharose)
Guaranteed Application *
WB
Suggested Dilutions
WB: 0.1-0.3ug/ml
Background
Related Pathway
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* OriGene provides validated application data and protocol, with money back guarantee.
HEK293 lysate (10ug protein in RIPA buffer) over expressing Human DAPP1 with DDK (FLAG) tag probed with TA311420 (0.1ug/ml) in Lane A and probed with anti-DDK tag (1:3000, Cat# TA50011-100) in Lane C. Mock-transfected HEK293 probed with TA311420 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
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