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Anti-SMPD2 Antibody EPR6718
Also for SMPD2 (NM_003080)
|A synthetic peptide corresponding to residues in human nSMase1 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, ASSAY, IHC
||ICC/IF: 1:250 - 1:500; WB: 1:1000 - 1:10000; IP: 1:10 - 1:100
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt or IHC-P.
|Homo sapiens sphingomyelin phosphodiesterase 2, neutral membrane (neutral sphingomyelinase) (SMPD2)|
|ISC1; NSMASE; NSMASE1|
|nSMase1 was initially identified as a sphingomyelinase based on sequence similarity between bacterial sphingomyelinases and a yeast protein. Subsequent studies showed that its biological function is less likely to be as a sphingomyelinase and instead as a lysophospholipase (1).|
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Western blot - Anti-nSMase antibody [EPR6718]; All lanes : Anti-nSMase antibody [EPR6718] at 1/1000 dilution.Lane 1 : 293T cell lysate.Lane 2 : MOLT4 cell lysate.Lane 3 : K562 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 48 kDa.
Western blot - Anti-nSMase antibody [EPR6718]; All lanes : Anti-nSMase antibody [EPR6718] at 1/1000 dilution.Lane 1 : Mouse brain lysate.Lane 2 : Rat brain lysates .Lysates/proteins at 10 µg per lane.Secondary.Goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 48 kDa.
Other-Anti-nSMase antibody [EPR6718](ab131330); Equilibrium disassociation constant (KD)..
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nSMase antibody [EPR6718]; Immunofluorescent analysis of U-87 MG cells labelling nSMase with ab131330 at 1/250 dilution.