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Home Antibody All anti-PRKAB1 antibodies

Anti-PRKAB1 Antibody EP345

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA311041
  • Rabbit Monoclonal antibody against AMPK beta 1 (PRKAB1)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC416773) , 20ug
100ul 325 3-7 Days
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WB(1)
FC(1)
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Also for PRKAB1 (NM_006253)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human AMPK beta 1 was used as an immunogen.
Clone NameEP345 IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, FC Suggested DilutionsWB: 1:1000 - 1:10000; FC: 1:10 - 1:100
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for ICC,IHC-P or IP.

Reference Data

Target NameHomo sapiens protein kinase, AMP-activated, beta 1 non-catalytic subunit (PRKAB1)
Alternative NameAMPK; HAMPKb
Database LinkNP_006244
FunctionThe 5-AMP-activated protein kinase (AMPK) is a member of the SNF1 (sucrose nonfermentor) kinase family (1). AMPK is a heterotrimeric protein comprising α (63 kDa), β (38 kDa) and γ (38 kDa) subunits (2). The alpha subunit is the catalytic subunit, while beta and gamma are noncatalytic subunits, although they have been found to interact with the active subunit in liver. AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA, as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase (3). AMPK beta-1 mediates the association of the AMPK heterotrimeric complex in vitro (2). Two isoforms have been found, and the difference in expression patterns indicates tissue-specific roles for these isoforms (4).
Related Pathway

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WB Image
Western blot - AMPK beta 1 antibody [EP345]; All lanes : Anti-AMPK beta 1 antibody [EP345] at 1/1000 dilution.Lane 1 : 293T cell lysate.Lane 2 : Fetal kidney lysate.Lane 3 : HeLa cell lysate.Lane 4 : NIH/3T3 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 2000 .Predicted band size : 38 kDa.Observed band size : 38 kDa.
FC Image
Flow Cytometry - AMPK beta 1 antibody [EP345]; Flow cytometric analysis of permeabilized HeLa cells using TA311041 at a 1/10 dilution (red), or a rabbit IgG (negative) (green).

 

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