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Anti-PRKAB1 Antibody EP345
Also for PRKAB1 (NM_006253)
|A synthetic peptide corresponding to residues in human AMPK beta 1 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; FC: 1:10 - 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for ICC,IHC-P or IP.
|Homo sapiens protein kinase, AMP-activated, beta 1 non-catalytic subunit (PRKAB1)|
|The 5-AMP-activated protein kinase (AMPK) is a member of the SNF1 (sucrose nonfermentor) kinase family (1). AMPK is a heterotrimeric protein comprising α (63 kDa), β (38 kDa) and γ (38 kDa) subunits (2). The alpha subunit is the catalytic subunit, while beta and gamma are noncatalytic subunits, although they have been found to interact with the active subunit in liver. AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA, as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase (3). AMPK beta-1 mediates the association of the AMPK heterotrimeric complex in vitro (2). Two isoforms have been found, and the difference in expression patterns indicates tissue-specific roles for these isoforms (4).|
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Western blot - AMPK beta 1 antibody [EP345]; All lanes : Anti-AMPK beta 1 antibody [EP345] at 1/1000 dilution.Lane 1 : 293T cell lysate.Lane 2 : Fetal kidney lysate.Lane 3 : HeLa cell lysate.Lane 4 : NIH/3T3 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 2000 .Predicted band size : 38 kDa.Observed band size : 38 kDa.
Flow Cytometry - AMPK beta 1 antibody [EP345]; Flow cytometric analysis of permeabilized HeLa cells using ab108250 at a 1/10 dilution (red), or a rabbit IgG (negative) (green).