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Anti-NR3C1 Antibody EPR4595
Also for NR3C1 (NM_000176)
|A recombinant protein fragment corresponding to amino acids 2-149 of human Glucocorticoid Receptor was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:50000 - 1:200000; FC: 1:100 - 1:1000
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for ICC,IHC-P or IP.
|Homo sapiens nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) (NR3C1), transcript variant 1|
|GCCR; GCR; GR; GRL|
|Glucocorticoid Receptor can act as both a transcription factor and as a regulator of other transcription factors. This protein can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. It is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Mutations in Glucocorticoid Receptor are a cause of glucocorticoid resistance, or cortisol, resistance (1).|
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Western blot - Glucocorticoid Receptor antibody [EPR4595]; All lanes : Anti-Glucocorticoid Receptor antibody [EPR4595] at 1/50000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : A431 cell lysate.Lane 3 : Jurkat cell lysate.Lane 4 : HepG2 cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 86 kDa.
Flow Cytometry - Anti-Glucocorticoid Receptor antibody [EPR4595]; Overlay histogram showing Jurkat cells stained with ab109022 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.