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Home Antibody All anti-KRT20 antibodies

Anti-KRT20 Antibody EPR1621(2)

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA310965
  • Rabbit Monoclonal antibody against Cytokeratin 20 (KRT20)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC402725) , 20ug
100ul 325 3-7 Days
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WB(1)
IF(1)
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Also for KRT20 (NM_019010)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human Cytokeratin 20 was used as an immunogen.
Clone NameEPR1621(2) IsotypeIgG
Species ReactivityHuman, Mouse, Rat ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IF Suggested DilutionsICC/IF: 1:50 - 1:100; WB: 1:10000 - 1:50000; IP: 1:10 - 1:100
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt or IHC-P.

Reference Data

Target NameHomo sapiens keratin 20 (KRT20)
Alternative NameCD20; CK-20; CK20; K20; KRT21
Database LinkNP_061883
FunctionIntermediate-sized filament (IF) protein designated cytokeratin (CK) 20 is a major cellular protein of mature enterocytes and goblet cells commonly found in mucosal epithelium of the mammalian gastrointestinal tract (1). Results strongly suggest that transcriptional regulation of keratin genes in the intestinal epithelium occurs at the level of both immature and terminally differentiated epithelial cells, and is tightly regulated during both fetal development and crypt-to-villus differentiation of the intestinal epithelium (2). CK20 has recently been reported to be useful to distinguish between primary and metastatic lung adenocarcinoma. CK20 expression was significantly more prevalent in adenocarcinoma that originated in the GI tract than that of pulmonary or breast origin (3).
Related Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Cytokeratin 20 antibody [EPR1621(2)]; All lanes : Anti-Cytokeratin 20 antibody [EPR1621(2)] at 1/10000 dilution.Lane 1 : SW480 lysate.Lane 2 : Mouse small intestine lysate.Lane 3 : Rat small intestine lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 48 kDa.Observed band size : 46 kDa .
IF Image
Immunocytochemistry/ Immunofluorescence - Cytokeratin 20 antibody [EPR1621(2)]; Immunofluorescent staining of Cytokeratin 20 in SW480 cells, using TA310965 at a 1/50 dilution.

 

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