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Anti-KRT20 Antibody EPR1621(2)
Also for KRT20 (NM_019010)
|A synthetic peptide corresponding to residues in human Cytokeratin 20 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||ICC/IF: 1:50 - 1:100; WB: 1:10000 - 1:50000; IP: 1:10 - 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt or IHC-P.
|Homo sapiens keratin 20 (KRT20)|
|CD20; CK-20; CK20; K20; KRT21|
|Intermediate-sized filament (IF) protein designated cytokeratin (CK) 20 is a major cellular protein of mature enterocytes and goblet cells commonly found in mucosal epithelium of the mammalian gastrointestinal tract (1). Results strongly suggest that transcriptional regulation of keratin genes in the intestinal epithelium occurs at the level of both immature and terminally differentiated epithelial cells, and is tightly regulated during both fetal development and crypt-to-villus differentiation of the intestinal epithelium (2). CK20 has recently been reported to be useful to distinguish between primary and metastatic lung adenocarcinoma. CK20 expression was significantly more prevalent in adenocarcinoma that originated in the GI tract than that of pulmonary or breast origin (3).|
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Western blot - Cytokeratin 20 antibody [EPR1621(2)]; All lanes : Anti-Cytokeratin 20 antibody [EPR1621(2)] at 1/10000 dilution.Lane 1 : SW480 lysate.Lane 2 : Mouse small intestine lysate.Lane 3 : Rat small intestine lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 48 kDa.Observed band size : 46 kDa .
Immunocytochemistry/ Immunofluorescence - Cytokeratin 20 antibody [EPR1621(2)]; Immunofluorescent staining of Cytokeratin 20 in SW480 cells, using ab109111 at a 1/50 dilution.