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Anti-THEMIS Antibody EPR7353
Also for THEMIS (NM_001010923)
|A synthetic peptide corresponding to residues in human THEMIS was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; ICC: 1:50 - 1:100; FC: 1:10 - 1:100
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
|Tissue culture supernatant
|Is unsuitable for IHC-P.
|Homo sapiens thymocyte selection associated (THEMIS), transcript variant 2|
|bA325O24.3; bA325O24.4; C6orf190; C6orf207; GASP; SPOT; TSEPA|
Entrez Gene 387357 Human
Entrez Gene 210757 Mouse
Entrez Gene 498985 Rat
|THEMIS plays a regulatory role in both positive and negative T-cell selection during late thymocyte development. It functions through T-cell antigen receptor signaling, and it is necessary for proper lineage commitment and maturation of T-cells (1).|
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Western blot - Anti-Themis antibody [EPR7353]; All lanes : Anti-Themis antibody [EPR7353] at 1/1000 dilution.Lane 1 : Jurkat cell lysate.Lane 2 : Human thymus lysates.Lysates/proteins at 10 ug per lane.Secondary.Goat anti-Rabbit HRP at 1/2000 dilution.Predicted band size : 73 kDa.
Flow Cytometry - Anti-Themis antibody [EPR7353]; TA310895 at 1/10 dilution staining Themis in permeabilized Jurkat cells by Flow cytometry (red) compared with a rabbit IgG negative control (green).