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Anti-SUPT5H Antibody EPR5145(2)
Also for SUPT5H (NM_001111020)
|A synthetic peptide corresponding to residues in human SUPT5H was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, ASSAY, IHC, IF
||WB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250; ICC/IF: 1:100 - 1:250
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
|Tissue culture supernatant
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens suppressor of Ty 5 homolog (S. cerevisiae) (SUPT5H), transcript variant 2|
|SPT5; SPT5H; Tat-CT1|
Entrez Gene 6829 Human
Entrez Gene 308472 Rat
|SUPT5H is a component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.|
|Transcription Factors |
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Western blot - Anti-SUPT5H antibody [EPR5145(2)]; All lanes : Anti-SUPT5H antibody [EPR5145(2)] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : HepG2 cell lysate.Lane 3 : RAW264.7 cell lysate.Lane 4 : PC12 cell lysate.Lane 5 : NIH 3T3 cell lysate.Lane 6 : Jurkat cell lysate.Lane 7 : 293T cell lysate.Lysates/proteins at 10 ug per lane.Secondary.Goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 121 kDa.
Other-Anti-SUPT5H antibody [EPR5145(2)](TA310887); Equilibrium disassociation constant (KD)..
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUPT5H antibody [EPR5145(2)]; TA310887 at 1/100 dilution staining SUPT5H in paraffin-embedded Human colon tissue by immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-SUPT5H antibody [EPR5145(2)]; TA310887 at 1/100 dilution staining SUPT5H in HeLa cells by immunofluorescence.