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Anti-SPHK1 Antibody EPR4293(2)
Also for SPHK1 (NM_001142601)
|A synthetic peptide corresponding to residues in human SPHK1 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; FC: 1:10 - 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for ICC,IHC-P or IP.
|Homo sapiens sphingosine kinase 1 (SPHK1), transcript variant 3|
|Sphingosine-1-phosphate (SPP) is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival, and extracellularly, it is a ligand for EDG1. Various stimuli increase cellular levels of SPP by activation of sphingosine kinase (SPHK), the enzyme that catalyzes the phosphorylation of sphingosine. Competitive inhibitors of SPHK block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors, including platelet-derived growth factor and serum (1).|
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Western blot - SPHK1 antibody [EPR4293(2)]; All lanes : Anti-SPHK1 antibody [EPR4293(2)] at 1/1000 dilution.Lane 1 : Raji cell lysate.Lane 2 : JAR cell lysate.Lane 3 : HUVEC cell lysate.Lane 4 : HT-1080 cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 43 kDa.Observed band size : 51 kDa .
Flow Cytometry - SPHK1 antibody [EPR4293(2)]; Flow cytometric analysis of permeabilized Raji cells using ab109522 (red), or a rabbit IgG (negative) (green).