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Anti-SMG5 Antibody EPR3503(2)
Also for SMG5 (NM_015327)
|A synthetic peptide corresponding to residues in human SMG5 was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250; ICC/IF: 1:100 - 1:250
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt or IP.|
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens SMG5 nonsense mediated mRNA decay factor (SMG5)|
|EST1B; LPTS-RP1; LPTSRP1; RP11-54H19.7; SMG-5|
|SMG5 is an essential factor in nonsense-mediated mRNA decay, a conserved pathway that degrades mRNAs with premature translation termination codons. Phosphorylated UPF1 is directly recognized by SMG5, SMG6 and SMG7 via a conserved 14-3-3 like domain present in all three proteins. These proteins are thought to serve as adaptors between phosphorylated UPF1 and protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation (1).|
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Western blot - Anti-SMG5 antibody [EPR3503(2)]; All lanes : Anti-SMG5 antibody [EPR3503(2)] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : 293T cell lysate.Lane 3 : K562 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-Rabbit HRP at 1/2000 dilution.Predicted band size : 114 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMG5 antibody [EPR3503(2)]; ab129107, at 1/100 dilution, staining SMG5 in Paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMG5 antibody [EPR3503(2)]; ab129107, at 1/100 dilution, staining SMG5 in Paraffin-embedded Human thyroid gland carcinoma tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-SMG5 antibody [EPR3503(2)]; ab129107, at 1/100 dilution, staining SMG5 in 293T cells by Immunofluorescence.