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Anti-ROCK2 Antibody EPR7141(B)


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SKU Description Amount Price Availability*  
  • Rabbit Monoclonal antibody against ROCK2
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC417653) , 20ug Explanation
100ul $325 3-7 Days
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Also for ROCK2 (NM_004850)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues at the C-terminus of human ROCK2 was used as an immunogen.
Clone NameEPR7141(B) IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, ASSAY, IHC Suggested DilutionsWB: 1:10000 - 1:50000; IHC-P: 1:50 - 1:100; ICC/IF: 1:100 - 1:250
Predicted MW Explanation 161 kDa
BufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens Rho-associated, coiled-coil containing protein kinase 2 (ROCK2)
Alternative NameROCK-II
Database LinkNP_004841
Entrez Gene 9475 Human
Entrez Gene 19878 Mouse
Entrez Gene 25537 Rat
FunctionROCK2 belongs to the protein kinase superfamily, and it is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1, is a target for the small GTPase Rho (1,2).
Related PathwayProtein KinaseDruggable Genome Chemokine signaling pathwayVascular smooth muscle contractionWnt signaling pathwayTGF-beta signaling pathwayAxon guidanceFocal adhesionMore Pathways >>

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* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - Anti-ROCK2 antibody [EPR7141(B)]; All lanes : Anti-ROCK2 antibody [EPR7141(B)] at 1/10000 dilution.Lane 1 : Hela cell lysate.Lane 2 : L6 cell lysate.Lane 3 : A673 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 161 kDa.
Other-Anti-ROCK2 antibody [EPR7141(B)](TA310825); Equilibrium disassociation constant (KD)..
Immunohistochemistry (Frozen sections) - Anti-ROCK2 antibody [EPR7141(B)]; TA310825 stainingROCK2 in Mouse hippocamus brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 10%serum for 60 minutes at 25°C. Samples were incubated with primary antibody (1/200 in PBS + 10% Goat serum + 2% Horse serum + 1% BSA) for 16 hours at 4°C. An Alexa Fluor?488-conjugatedGoat anti-rabbit polyclonal (1/1000) was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 antibody [EPR7141(B)]; TA310825, at 1/50 dilution, staining ROCK2 in Paraffin-embedded Human kidney tissue by Immunohistochemistry.


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