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Anti-PSMA1 Antibody EPR5451
Also for PSMA1 (NM_001143937)
|A synthetic peptide corresponding to residues in human PSMA1 was used as an immunogen.|
|Human, Mouse, Rat
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:250 - 1:500; ICC: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for Flow Cyt.
|Homo sapiens proteasome (prosome, macropain) subunit, alpha type, 1 (PSMA1), transcript variant 3|
|HC2; HEL-S-275; NU; PROS30|
|The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMA1 is a member of the peptidase T1A family, that is a 20S core alpha subunit (1).|
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Western blot - Proteasome 20S C2 antibody [EPR5451]; All lanes : Anti-Proteasome 20S C2 antibody [EPR5451] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : Jurkat cell lysate.Lane 3 : HepG2 cell lysate.Lane 4 : PC-3 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.Standard HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 30 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Proteasome 20S C2 antibody [EPR5451]; Immunohistochemical analysis of paraffin-embedded Human breast tissue using TA310786 at 1/250 dilution.