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Anti-PRPF19 Antibody EPR7446
Also for PRPF19 (NM_014502)
|A synthetic peptide corresponding to residues in human PRP19 was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, ASSAY, IHC
||WB: 1:1000 - 1:10000; IHC-P: 1:50 - 1:100; ICC: 1:250 - 1:500; FC: 1:100 - 1:500
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
|Tissue culture supernatant
|Is unsuitable for IP.
|Homo sapiens pre-mRNA processing factor 19 (PRPF19)|
|hPSO4; NMP200; PRP19; PSO4; SNEV; UBOX4|
Entrez Gene 27339 Human
Entrez Gene 28000 Mouse
Entrez Gene 246216 Rat
|PRP19, an essential RNA splicing factor, is a core component of the spliceosomal NinTeen associated-Complex (NTC), a sub-complex required for the activation of the spliceosome. It plays a direct role in the activation and structural stabilization of the spliceosome. PRP19 utilizes its N-terminal U-box domain to recruit Ubc3, and possibly other E2 conjugating enzymes (1). In addition, it is involved in DNA double-strand break (DSB) repair and pre-mRNA splicing reaction. PRP19 binds double-stranded DNA in a sequence-nonspecific manner (2).|
|Druggable Genome SpliceosomeUbiquitin mediated proteolysis|
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Western blot - Anti-PRP19 antibody [EPR7446]; All lanes : Anti-PRP19 antibody [EPR7446] at 1/1000 dilution.Lane 1 : HepG2 cell lysate.Lane 2 : A431 cell lysate.Lane 3 : MCF7 cell lysate.Lane 4 : PC12 cell lysate.Lane 5 : NIH 3T3 cell lysate.Lane 6 : HeLa cell lysate.Lane 7 : K562 cell lysate.Lane 8 : U937 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.Goat anti-Rabbit HRP at 1/2000 dilution.Predicted band size : 55 kDa.
Other-Anti-PRP19 antibody [EPR7446](TA310784); Equilibrium disassociation constant (KD)..
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRP19 antibody [EPR7446]; TA310784, at 1/50 dilution, staining PRP19 in Paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRP19 antibody [EPR7446]; TA310784, at 1/50 dilution, staining PRP19 in Paraffin-embedded Human colon carcinoma tissue by Immunohistochemistry.