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Home Antibody All anti-PRMT5 antibodies

Anti-PRMT5 Antibody EPR5772

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA310782
  • Rabbit Monoclonal antibody against PRMT5
100ul 325 3-7 Days
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WB(1)
IHC(1)
IF(1)
FC(1)
Assay(1)
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Also for PRMT5 (NM_001039619)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human PRMT5 was used as an immunogen.
Clone NameEPR5772 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, ASSAY, IHC, IF, FC Suggested DilutionsWB: 1:10000 - 1:50000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250; FC: 1:500 - 1:1000
Predicted MW Explanation 73 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens protein arginine methyltransferase 5 (PRMT5), transcript variant 2
Alternative NameHRMT1L5; IBP72; JBP1; SKB1; SKB1Hs
Database LinkNP_001034708
Entrez Gene 10419 Human
Entrez Gene 27374 Mouse
Entrez Gene 364382 Rat
FunctionPRMT5 is an arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. It specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. PRMT5 plays a role in the assembly of snRNP core particles as well as in cytokine-activated transduction pathways. It negatively regulates cyclin E1 promoter activity and cellular proliferation (1).
Related PathwayStem cell - Pluripotency

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - PRMT5 antibody [EPR5772]; All lanes : Anti-PRMT5 antibody [EPR5772] at 1/10000 dilution.Lane 1 : 293T cell lysate.Lane 2 : HepG2 cell lysate.Lane 3 : HeLa cell lysate.Lane 4 : NIH3T3 cell lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 73 kDa.
Assay Image
Other-Anti-PRMT5 antibody [EPR5772](TA310782); Equilibrium disassociation constant (KD)..
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PRMT5 antibody [EPR5772]; TA310782 at 1/100 dilution staining PRMT5 in Mouse Kidney by Immunohistochemistry, Paraffin-embedded tissue.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-PRMT5 antibody [EPR5772]; ICC/IF image of TA310782 stainedHepG2 cells. The cells were 100%methanol fixed (5 min) and then incubated in 1%BSA / 10% normalgoat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was , DyLight? 488goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
FC Image
Flow Cytometry-Anti-PRMT5 antibody [EPR5772](TA310782); Overlay histogram showing HeLa cells stained with TA310782 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.

 

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