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Home Antibody All anti-PRMT5 antibodies

Anti-PRMT5 Antibody EPR5772

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA310782 Rabbit Monoclonal antibody against PRMT5 100ul $325 In Stock
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WB(1)
IHC(1)
IF(1)
FC(1)
Assay(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human PRMT5 was used as an immunogen.
Clone NameEPR5772 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, Assay, IHC, IF, FC Suggested DilutionsWB: 1:10000 - 1:50000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250; FC: 1:500 - 1:1000
Predicted MW Explanation 73 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens protein arginine methyltransferase 5 (PRMT5), transcript variant 2
Alternative NameHRMT1L5; IBP72; JBP1; SKB1; SKB1Hs
Database LinkNP_001034708
Entrez Gene 10419 Human
Entrez Gene 27374 Mouse
Entrez Gene 364382 Rat
FunctionPRMT5 is an arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. It specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. PRMT5 plays a role in the assembly of snRNP core particles as well as in cytokine-activated transduction pathways. It negatively regulates cyclin E1 promoter activity and cellular proliferation (1).
Related PathwayStem cell - Pluripotency

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - PRMT5 antibody [EPR5772]; All lanes : Anti-PRMT5 antibody [EPR5772] at 1/10000 dilution.Lane 1 : 293T cell lysate.Lane 2 : HepG2 cell lysate.Lane 3 : HeLa cell lysate.Lane 4 : NIH3T3 cell lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 73 kDa.
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Other-Anti-PRMT5 antibody [EPR5772](TA310782); Equilibrium disassociation constant (KD)..
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PRMT5 antibody [EPR5772]; TA310782 at 1/100 dilution staining PRMT5 in Mouse Kidney by Immunohistochemistry, Paraffin-embedded tissue.
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ICC/IF image of TA310782 stainedHepG2 cells. The cells were incubated with the antibody overnight at 4.. The secondary antibody (green) was , DyLight? 488goat anti-rabbit IgG (H+L) used at 1:250. for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at 1:200 for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
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Flow Cytometry-Anti-PRMT5 antibody(TA310782); Overlay histogram showing HeLa cells stained with TA310782 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit IgG (monclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.
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