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Anti-PRKDC PHOSPHO Antibody EPR5670
Also for PRKDC (NM_006904)
|A phospho specific peptide corresponding to residues surrounding Serine 2056 of human DNA-PK/PRKDC was used as an immunogen. This antibody only detects DNA-PK/PRKDC phosphorylated at Serine 2056.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
|WB, ASSAY, IHC
||WB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250
|Does not react with Mouse, Rat. Is unsuitable for ICC or IP.|
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA (Protein A or G Sepharose)
|Is unsuitable for ICC or IP.
|Homo sapiens protein kinase, DNA-activated, catalytic polypeptide (PRKDC), transcript variant 1|
|DNA-PKcs; DNAPK; DNPK1; HYRC; HYRC1; p350; XRCC7|
|DNA-activated serine/threonine protein kinase (DNA-PK) is a nuclear protein kinase that is involved in DNA nonhomologous end joining (NHEJ) required for double- strand brake repair and V(D)J recombination. DNA-PK is composed of a large catalytic subunit of ~470 kDa (DNA-PKcs) and a DNA binding heterodimer of approximately 70- and 80-kDa subunits called Ku. (1-2). Autophosphorylation of DNA-PK correlates with loss of protein kinase activity and dissociation of the DNA-PKcs-Ku complex. DNA-PK phosphorylates Hsp90, p53 and c-Jun (3-4).|
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Western blot - Anti-DNA PK (phospho S2056) antibody [EPR5670]; All lanes : Anti-DNA PK (phospho S2056) antibody [EPR5670] at 1/1000 dilution.Lane 1 : Jurkat cell lysate, untreated.Lane 2 : Jurkat cell lysate, treated with alkaline phosphatase.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-Rabbit HRP at 1/2000 dilution.Predicted band size : 469 kDa.
Other-Anti-DNA PK (phospho S2056) antibody [EPR5670](ab124918); Equilibrium disassociation constant (KD)..
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA PK (phospho S2056) antibody [EPR5670]; ab124918, at 1/100 dilution, staining DNA PK in paraffin embedded Human brain tissue by immunohistochemistry.