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Anti-MTOR Antibody EPR390(N)
Also for MTOR (NM_004958)
|A synthetic peptide corresponding to residues near the N-terminus of human mTOR/FRAP was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|Preservative: 0.01% Sodium azide|
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
|Homo sapiens mechanistic target of rapamycin (serine/threonine kinase) (MTOR)|
|FRAP; FRAP1; FRAP2; RAFT1; RAPT1|
|The mammalian target of rapamycin (mTOR) is a major effector of cell growth and proliferation via the regulation of protein synthesis. mTOR is composed of an N-term; 20 tandem repeats- HEAT which are implicated in protein-protein interactions and a C-term; which includes a FAT domain, a FBR domain, a kinase domain, a NDR domain and a FATC domain. The FATC domain is essential to mTOR activity and the deletion of a single amino acid from this domain abrogates the activity. mTOR can be auto-phosphorylated via its intrinsic serine/threonine kinase activity. mTOR regulates protein synthesis through the phosphorylation and inactivation of the repressor of mRNA translation 4E-BP1 and through the phosphorylation and activation of S6 kinase. RAPTOR (regulatory associated protein of TOR) is a positive regulator of TOR. Other known mediators of mTOR include PI3-K and ATK from the insulin pathway (1).|
Senescence and Autophagy
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Western blot - Anti-mTOR antibody [EPR390(N)]; All lanes : Anti-mTOR antibody [EPR390(N)] at 1/10000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : K562 cell lysate.Lane 3 : Raji cell lysate.Lane 4 : HepG2 cell lysate.Lane 5 : 293T cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP-conjugated goat anti-rabbit polyclonal IgG at 1/2000 dilution.Predicted band size : 289 kDa.Observed band size : 289 kDa.