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Anti-MCM3 Antibody EPR7081
Also for MCM3 (NM_001270472)
|A synthetic peptide corresponding to residues in human MCM3 was used as an immunogen.|
||Tissue culture supernatant
||Lot dependent; please refer to CoA along with shipment
|WB, ASSAY, FC
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; FC: 1:10 - 1:100
|Does not react with Mouse, Rat. Is unsuitable for ICC/IF or IHC-P.|
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
|Is unsuitable for ICC/IF or IHC-P.
|Homo sapiens minichromosome maintenance complex component 3 (MCM3), transcript variant 2|
|HCC5; P1-MCM3; P1.h; RLFB|
|MCM3 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and the recruitment of other DNA replication related proteins. MCM3 is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. MCM3 also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression (1).|
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Western blot - Anti-MCM3 antibody [EPR7081]; All lanes : Anti-MCM3 antibody [EPR7081] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : K562 cell lysate.Lane 3 : 293T cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 91 kDa.
Other-Anti-MCM3 antibody [EPR7081](TA310680); Equilibrium disassociation constant (KD)..
Flow Cytometry - Anti-MCM3 antibody [EPR7081]; TA310680, at 1/10 dilution staining MCM3 in permeabilized 293T cells by Flow Cytometry (red). A rabbit IgG negative control is shown in green.