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Anti-MAP4K4 Antibody EPR1642(2)
Also for MAP4K4 (NM_001242559)
|A synthetic peptide corresponding to residues on the C-terminus in human MAP4K4 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; FC: 1:1800
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant
|Is unsuitable for ICC,IHC-P or IP.
|Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 4|
|FLH21957; HEL-S-31; HGK; MEKKK4; NIK|
|MAP4K4 is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway.|
MAPK signaling pathway
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Western blot - Anti-MAP4K4 antibody [EPR1642(2)]; All lanes : Anti-MAP4K4 antibody [EPR1642(2)] at 1/1000 dilution.Lane 1 : 293T cell lysate.Lane 2 : Jurkat cell lysate.Lane 3 : SH-SY5Y cell lysate.Lysates/proteins at 10 µg/ml per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 142 kDa.
Flow Cytometry - Anti-MAP4K4 antibody [EPR1642(2)]; Flow Cytometry analysis of permeabilized Jurkat cells labelling MAP4K4 with TA310673 at 1/800 dilution (red). Rabbit IgG (negative control) shown in green.