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Anti-MAP1LC3A Antibody EP1528Y
Also for MAP1LC3A (NM_032514)
|A synthetic peptide corresponding to residues on the N-terminus of human MAPILC3A was used as an immogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1,000 - 10,000; IHC 1:100 - 250; ICC: 1:250 - 500; IP: 1:30;
|Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.|
|Homo sapiens microtubule-associated protein 1 light chain 3 alpha (MAP1LC3A), transcript variant 1|
|ATG8E; LC3; LC3A; MAP1ALC3; MAP1BLC3|
|Microtubule-associated MAPILC3A constitute nearly half of the mass of all the microtubule associated proteins that copurify with brain microtubules. MAPILC3A is composed of a heavy chain and multiple light-chain subunits. Amino acid sequencing of a 120 kd microtubule-binding and light chain-binding fragment of the heavy chain reveals that light chain 1 binds near the heavy chain N-terminus. These data indicate that the heavy chain and light chain 1 are produced by proteolytic processing of a MAP1B polyprotein and form a complex microtubule-binding domain (1). There are three human orthologs of the rat Map1LC3, named MAP1LC3A, MAP1LC3B, and MAP1LC3C. The three isoforms of human MAP1LC3 exhibit distinct expression patterns in different human tissues and also differ in their post-translation modifications. MAP1LC3A and MAP1LC3C are produced by the proteolytic cleavage after the conserved C-terminal Gly residue; MAP1LC3B does not undergo C-terminal cleavage and exists in a single modified form (2).|
Senescence and Autophagy
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Western blot - MAP1LC3A antibody; Anti-MAP1LC3A antibody [EP1528Y] at 1/50000 dilution + Fetal brain lysate at 10 µg.Secondary.goat anti-rabbit HRP labelled at 1/2000 dilution.Predicted band size : 14 kDa.Observed band size : 16 kDa .
Immunohistochemistry (Paraffin-embedded sections) - MAP1LC3A antibody; ab52628, at a 1/50 dilution, staining Human MAPILC3A in brain using Immunohistochemistry, Paraffin Embedded Tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-MAP1LC3A antibody [EP1528Y](ab52628); IHC image of ab52628 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab52628, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.