OriGene Technologies, Inc.
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-MAP1LC3A antibodies

Anti-MAP1LC3A Antibody EP1528Y

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA310672
  • Rabbit Monoclonal antibody against MAPILC3A (N-term)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC403169) , 20ug
100ul 325 3-7 Days
Add to Shopping Cart
WB(1)
IHC(2)
spacer
Also for MAP1LC3A (NM_032514)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues on the N-terminus of human MAPILC3A was used as an immogen.
Clone NameEP1528Y IsotypeIgG
Species ReactivityHuman, Mouse, Rat ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:1,000 - 10,000; IHC 1:100 - 250; ICC: 1:250 - 500; IP: 1:30;
BufferStore at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.

Reference Data

Target NameHomo sapiens microtubule-associated protein 1 light chain 3 alpha (MAP1LC3A), transcript variant 1
Alternative NameATG8E; LC3; LC3A; MAP1ALC3; MAP1BLC3
Database LinkNP_115903
FunctionMicrotubule-associated MAPILC3A constitute nearly half of the mass of all the microtubule associated proteins that copurify with brain microtubules. MAPILC3A is composed of a heavy chain and multiple light-chain subunits. Amino acid sequencing of a 120 kd microtubule-binding and light chain-binding fragment of the heavy chain reveals that light chain 1 binds near the heavy chain N-terminus. These data indicate that the heavy chain and light chain 1 are produced by proteolytic processing of a MAP1B polyprotein and form a complex microtubule-binding domain (1). There are three human orthologs of the rat Map1LC3, named MAP1LC3A, MAP1LC3B, and MAP1LC3C. The three isoforms of human MAP1LC3 exhibit distinct expression patterns in different human tissues and also differ in their post-translation modifications. MAP1LC3A and MAP1LC3C are produced by the proteolytic cleavage after the conserved C-terminal Gly residue; MAP1LC3B does not undergo C-terminal cleavage and exists in a single modified form (2).
Related Pathway
Senescence and Autophagy

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - MAP1LC3A antibody; Anti-MAP1LC3A antibody [EP1528Y] at 1/50000 dilution + Fetal brain lysate at 10 µg.Secondary.goat anti-rabbit HRP labelled at 1/2000 dilution.Predicted band size : 14 kDa.Observed band size : 16 kDa .
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - MAP1LC3A antibody; TA310672, at a 1/50 dilution, staining Human MAPILC3A in brain using Immunohistochemistry, Paraffin Embedded Tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-MAP1LC3A antibody [EP1528Y](TA310672); IHC image of TA310672 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with TA310672, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title