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Anti-IFITM3 Antibody EPR5242
Also for IFITM3 (NM_021034)
|A synthetic peptide corresponding to residues in human IFITM3 was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:1000 - 1:10000; IHC-P: 1:250 - 1:500; ICC: 1:100 - 1:250
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens interferon induced transmembrane protein 3 (IFITM3), transcript variant 1|
|1-8U; DSPA2b; IP15|
|IFITM3 is a member of the IFITM family, which mediates cellular processes, including the homotypic cell adhesion functions of interferons (IFNs) (1). It is an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus (WNV), by inhibiting the early steps of replication (2). High expression levels of IFITM3 have been found in gastric cancer cell and colorectal tumors (1).|
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Western blot - Fragilis antibody [EPR5242]; All lanes : Anti-Fragilis antibody [EPR5242] at 1/1000 dilution.Lane 1 : HeLa cell lysate .Lane 2 : 293T cell lysate .Lane 3 : Human placenta lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 15 kDa.
Western blot - Anti-Fragilis antibody [EPR5242]; Anti-Fragilis antibody [EPR5242] at 1/1000 dilution + Human Fragilis full length protein at 0.01 µg.Secondary.Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution.developed using the ECL technique.Performed under reducing conditions.Exposure time : 10 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Fragilis antibody [EPR5242]; Immunohistochemical analysis of Fragilis in paraffin-embedded Human kidney tissue using ab109429 at 1/250 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Fragilis antibody [EPR5242]; ICC/IF image of ab109429 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab109429 at 1/50 dilution overnight at +4°C. The secondary antibody (green)was DyLight? 488 goat anti- rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.