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Anti-GLA Antibody EPR5829
|A synthetic peptide corresponding to residues at the C-terminus of human GLA was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, ASSAY, FC
||WB: 1:1000 - 1:10000; FC: 1:100 - 1:500
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for ICC,IHC-P or IP.
|Homo sapiens galactosidase, alpha (GLA)|
|GLA is a homodimeric glycoprotein that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. This enzyme predominantly hydrolyzes ceramide trihexoside, and it can catalyze the hydrolysis of melibiose into galactose and glucose. A variety of mutations in this GLA affect the synthesis, processing, and stability of this enzyme, which causes Fabry disease, a rare lysosomal storage disorder that results from a failure to catabolize alpha-D-galactosyl glycolipid moieties (1).|
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Western blot - Anti-Galactosidase alpha antibody [EPR5829]; All lanes : Anti-Galactosidase alpha antibody [EPR5829] at 1/1000 dilution.Lane 1 : 293T cell lysate.Lane 2 : A431 cell lysate.Lane 3 : MCF7 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 49 kDa.
Other-Anti-Galactosidase alpha antibody [EPR5829](ab129173); Equilibrium disassociation constant (KD)..
Flow Cytometry - Anti-Galactosidase alpha antibody [EPR5829]; ab129173 (red), at a 1/100 dilution, staining Galactosidase alpha in permeabilized MCF7 cells by Flow Cytometry. A rabbit IgG was used as a negative control (green).