Home Antibody All anti-ELANE antibodies
Anti-ELANE Antibody EPR7479
Also for ELANE (NM_001972)
|A synthetic peptide corresponding to residues at the C-terminus of human Neutrophil Elastase was used as an immunogen.|
||Tissue culture supernatant
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
|WB, ASSAY, IHC, FC
||WB: 1:1000 - 1:10000; IHC-P: 1:250 - 1:500; FC: 1:10 - 1:100; ICC/IF: 1:250 - 1:500
|Does not react with Mouse, Rat. Is unsuitable for IP.|
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
|Is unsuitable for IP.
|Homo sapiens elastase, neutrophil expressed (ELANE)|
|ELA2; GE; HLE; HNE; NE; PMN-E; SCN1|
Entrez Gene 1991 Human
|Neutrophil Elastase hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils. The enzyme may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. Neutrophil Elastase degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this protein are associated with cyclic neutropenia and severe congenital neutropenia (SCN) (1). |
|TransmembraneProtease Systemic lupus erythematosus|
* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.
Western blot - Anti-Neutrophil Elastase antibody [EPR7479]; Anti-Neutrophil Elastase antibody [EPR7479] at 1/1000 dilution + HL60 cell lysate at 10 ug.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 29 kDa.
Other-Anti-Neutrophil Elastase antibody [EPR7479](TA310453); Equilibrium disassociation constant (KD)..
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neutrophil Elastase antibody [EPR7479]; Immunohistochemical analysis of paraffin-embedded Human bone marrow tissue labelling Neutrophil Elastase with TA310453 at 1/250 dilution.
Flow Cytometry - Anti-Neutrophil Elastase antibody [EPR7479]; Overlay histogram showing HL60 cells stained with TA310453 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.