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Home Antibody All anti-ELANE antibodies

Anti-ELANE Antibody EPR7479


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA310453 Rabbit Monoclonal antibody against Neutrophil Elastase (ELANE) 100ul $325 In Stock
LC419618 ELANE HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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OriGene Data

ImmunogenA synthetic peptide corresponding to residues at the C-terminus of human Neutrophil Elastase was used as an immunogen.
Clone NameEPR7479 IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, Assay, IHC, FC Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:250 - 1:500; FC: 1:10 - 1:100; ICC/IF: 1:250 - 1:500
BufferpH: 7.20; Preservative: 0.01% Sodium azide; Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Purification Protein A purified
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens elastase, neutrophil expressed (ELANE)
Alternative NameELA2; GE; HLE; HNE; NE; PMN-E; SCN1
Database LinkNP_001963
Entrez Gene 1991 Human
FunctionNeutrophil Elastase hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils. The enzyme may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. Neutrophil Elastase degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this protein are associated with cyclic neutropenia and severe congenital neutropenia (SCN) (1).
Related PathwayTransmembraneProtease Systemic lupus erythematosus

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - Anti-Neutrophil Elastase antibody [EPR7479]; Anti-Neutrophil Elastase antibody [EPR7479] at 1/1000 dilution + HL60 cell lysate at 10 ug.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 29 kDa.
Other-Anti-Neutrophil Elastase antibody [EPR7479](TA310453); Equilibrium disassociation constant (KD)..
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neutrophil Elastase antibody [EPR7479]; Immunohistochemical analysis of paraffin-embedded Human bone marrow tissue labelling Neutrophil Elastase with TA310453 at 1/250 dilution.
Flow Cytometry - Anti-Neutrophil Elastase antibody; Overlay histogram showing HL60 cells stained with TA310453 (red line). The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1:2000. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control.


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