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Anti-DCTN2 Antibody EPR5094
Also for DCTN2 (NM_006400)
|A synthetic peptide corresponding to residues in human DCTN2 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:10000 - 1:50000; IP: 1:10 - 1:100; FC: 1:10 - 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for ICC or IHC-P.
|Homo sapiens dynactin 2 (p50) (DCTN2), transcript variant 1|
|DCTN50; DYNAMITIN; RBP50|
|DCTN2 is a subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kDa. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. DCTN2 contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place.|
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Western blot - Dynamitin antibody [EPR5094]; All lanes : Anti-Dynamitin antibody [EPR5094] at 1/10000 dilution.Lane 1 : MCF7 cell lysate.Lane 2 : A375 cell lysate.Lane 3 : Saos2 cell lysate.Lane 4 : SH Y5Y cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 44 kDa.
Flow Cytometry - Dynamitin antibody [EPR5094]; ab109752 at 1/10 dilution staining Dynamitin in Saos2 cells by Flow cytometry (red) or a rabbit IgG (negative) (green).