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Anti-DBF4 Antibody EPR5635
Also for DBF4 (NM_006716)
|A synthetic peptide corresponding to residues in human DBF4 was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:10000 - 1:50000
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
|Homo sapiens DBF4 homolog (S. cerevisiae) (DBF4)|
|ASK; CHIF; DBF4A; ZDBF1|
|DBF4 is a regulatory protein that is required for Cdc7 kinase activation and forms a stable two-subunit complex with Cdc7. A two-subunit serine/threonine kinase, Cdc7-DBF4, is required for initiating DNA replication in all eukaryotes. Cdc7 is expressed at constant levels during the cell cycle, but DBF4 levels are cyclical and peak near the G1/S phase transition. Cdc7-DBF4 is recruited to replication origins to promote DNA synthesis by activating the MCM helicase (1).|
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Western blot - Anti-DBF4 antibody [EPR5635]; All lanes : Anti-DBF4 antibody [EPR5635] at 1/10000 dilution.Lane 1 : PC-3 cell lysates.Lane 2 : Jurkat cell lysates.Lane 3 : SW480 cell lysates.Lane 4 : HepG2 cell lysates.Lysates/proteins at 10 µg per lane.Predicted band size : 77 kDa.
Other-Anti-DBF4 antibody [EPR5635](ab124707); Equilibrium disassociation constant (KD)..