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Anti-CTCF Antibody EPR7314(B)
Also for CTCF (NM_001191022)
|A synthetic peptide corresponding to residues in human CTCF was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, ASSAY, IHC, IF, FC
||WB: 1:1000 - 1:10000; IHC-P: 1:250 - 1:500; FC: 1:100 - 1:1000; ICC/IF: 1:250 - 1:500
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
|Tissue culture supernatant
|Is unsuitable for IP.
|Homo sapiens CCCTC-binding factor (zinc finger protein) (CTCF), transcript variant 2|
Entrez Gene 10664 Human
Entrez Gene 13018 Mouse
Entrez Gene 83726 Rat
|CTCF is a member of the BORIS + CTCF family and is a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in CTCF have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors (1).|
|Transcription Factors |
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Western blot - Anti-CTCF antibody [EPR7314(B)]; All lanes : Anti-CTCF antibody [EPR7314(B)] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : 293T cell lysate.Lysates/proteins at 10 ug per lane.Secondary.Goat anti-Rabbit HRP at 1/2000 dilution.Predicted band size : 83 kDa.
Other-Anti-CTCF antibody [EPR7314(B)](TA310368); Equilibrium disassociation constant (KD)..
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTCF antibody [EPR7314(B)]; TA310368 at 1/250 dilution staining CTCF in paraffin embedded Human colon tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-CTCF antibody [EPR7314(B)]; TA310368 at 1/250 dilution staining CTCF in HeLa cells by Immunofluorescence.
Flow Cytometry - Anti-CTCF antibody [EPR7314(B)]; Overlay histogram showing Hela cells stained with TA310368 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.