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Anti-Chk1 PHOSPHO Antibody EPR765


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SKU Description Amount Price Availability*  
TA310325 Rabbit Monoclonal antibody against Chk1, pS280 (Phospho-specific) 100ul $325 In Stock
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OriGene Data

ImmunogenA phospho-specific peptide corresponding to residues surrounding Serine 280 of human Chk1 was used as an immunogen. The antibody only detects Chk1 phosphorylated on Serine 280.
Clone NameEPR765 IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB Suggested DilutionsWB: 1:5000 - 10,000; IHC 1:100 - 250;
BufferStore at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.

Reference Data

Target NameHomo sapiens checkpoint kinase 1 (CHEK1), transcript variant 3
Alternative NameCHK1
Database LinkNP_001265
Entrez Gene 1111 Human
FunctionChk1 is an evolutionarily conserved protein kinase that has been implicated in cell cycle checkpoint control in lower eukaryotes. Chk1 plays an essential role in the mammalian DNA damage checkpoint, embryonic development, and tumor suppression (1). It is activated in response to replication blocks and genotoxic stress, via phosphorylation of Serine 317 and Serine 345 (2). Activated Chk1 can bind to, phosphorylate (at Serine 216) and inactivate cdc25c (3, 4). Chk1 is phosphorylated by ATR in response to ultraviolet irradiation and inhibition of DNA replication, and by ATM in response to ionizing irradiation, resulting in enhanced kinase activity. Chk1 is phosphorylated at Serine 280 by AKT1/PKB, which is thought to promote mono and/or diubiquitination (5).
Related PathwayProtein KinaseStem cell - PluripotencyDruggable Genome Cell cyclep53 signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Chk1 (phospho S280) antibody [EPR765]; All lanes : Anti-Chk1 (phospho S280) antibody [EPR765] at 1/2500 dilution.Lane 1 : 293T cell lysate.Lane 2 : 293T cell lysate treated with Calyculin A.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 54 kDa.


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