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Home Antibody All anti-CAMK2A antibodies

Anti-CAMK2A PHOSPHO Antibody EPR1830

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA310229
  • Rabbit Monoclonal antibody against CaMKII, pT286 (CAMK2A) (Phospho-specific)
100ul $325 3-7 Days
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WB(1)
Assay(1)
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Also for CAMK2A (NM_015981)
cDNA Clone shRNA/siRNA Primer Pair Protein Antibody

OriGene Data

ImmunogenA phospho specific peptide corresponding to residues surrounding Threonine 286 of human CaMKII was used as an immunogen. This antibody only detects CaMKII phosphorylated at Threonine 286.
Clone NameEPR1830 IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, ASSAY Suggested DilutionsWB: 1:1000 - 1:10000
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for ICC,IHC-P or IP.

Reference Data

Target NameHomo sapiens calcium/calmodulin-dependent protein kinase II alpha (CAMK2A), transcript variant 1
Alternative NameCAMKA
Database LinkNP_057065
FunctionCalcium/calmodulin-dependent protein kinase II (CaMKII) is a widely distributed protein kinase that regulates numerous physiological functions. It is a ubiquitous serine/threonine protein kinase which is abundant in brain as a major PSD constituent. This enzyme is composed of distinct but related subunits (alpha, beta, gamma, and delta) (1). The synapse contains densely localized and interacting proteins that enable it to adapt to changing inputs. A Ca2+-sensitive protein complex is involved in the regulation of AMPA receptor synaptic plasticity. The complex is comprised of MUPPI, a multi-PDZ domain-containing protein; SynGAP, a synaptic GTPase-activating protein; and the Ca2+/calmodulin-dependent kinase CaMKII. In synapses of hippocampal neurons, SynGAP and CaMKII are brought together by direct physical interaction with the PDZ domains of MUPP1, and in this complex, SynGAP is phosphorylated. Ca2+CaM binding to CaMKII dissociates it from the MUPP1 complex, and Ca2+ entering via the NMDAR drives the dephosphorylation of SynGAP (2). The most important autophosphorylation site is Threonine 286, which resides in the vicinity of the autoinhibitory domain (3).
Related Pathway
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Melanogenesis
TGF Beta Signaling Pathway
Wnt Signaling Pathway

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WB Image
Western blot - Anti-CaMKII alpha (phospho T286) antibody [EPR1830]; All lanes : Anti-CaMKII alpha (phospho T286) antibody [EPR1830] at 1/1000 dilution.Lane 1 : Rat brain lysate untreated.Lane 2 : Rat brain lysate treated with Lambda Phosphatase.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 54 kDa.
Assay Image
Other-Anti-CaMKII alpha (phospho T286) antibody [EPR1830](TA310229); Equilibrium disassociation constant (KD)..

 

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