Anti-ABCA1 Antibody, EPR2485

Also for ABCA1 (NM_005502)

OriGene Data

Immunogen	A phospho specific peptide corresponding to residues surrounding serine 2054 of human ABCA1 was used as an immunogen. This antibody only detects ABCA1 phosphorylated at serine 2054.
Clone Name	EPR2485	Isotype
Species Reactivity	Human, Mouse	Concentration
Guaranteed Application *	WB, IF	Suggested Dilutions	WB: 1:1,000-10,000; IHC 1:50 - 100; FC: 1:10 - 100;
Buffer	Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Purification	(Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens ATP-binding cassette, sub-family A (ABC1), member 1 (ABCA1)
Alternative Name	ABC-1; ABC1; CERP; HDLDT1; TGD
Database Link	NP_005493
Function	ABCA1 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes, and are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. With cholesterol as its substrate, ABCA1 functions as a cholesterol efflux pump in the cellular lipid removal pathway. Mutations in this protein have been associated with Tangier's disease and familial high-density lipoprotein deficiency (1). It has been shown that Ser-1042 and Ser-2054, located in the nucleotide binding domains of ABCA1, are major phosphorylation sites for PKA. Phosphorylation of Ser-2054 within the nucleotide binding domain 2 was found to be particularly important for ApoA-I-dependent phospholipid efflux activity mediated by ABCA1 (2).
Related Pathway

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WB Image

Western blot analysis on SH-SY5Y cell lysates using anti-Phospho-ABCA1 (pS2054) RabMAb. Cells were either (A) untreated (B) treated with Okadaic Acid and Calyculin A.<br>Note: Primary antibody was diluted with 1% BSA.

IF Image

Immunofluorescent analysis of SH-SY5Y cells treated with Okadaic Acid and Calyculin A using anti-Phospho-ABCA1 (pS2054) RabMAb

Conjugation Services
OriGene provides conjugation services of TrueMAB antibodies with the following molecules:

HRP
Biotin
PE
FITC
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DyLight® 550
DyLight® 594
DyLight® 633

* DyLight is a trademark of Thermo-Fisher Scientific

Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.

Anti-ABCA1 Phospho Antibody EPR2485

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data