The antiserum was produced against synthesized phosphopeptide derived from humanPaxillin around the phosphorylation site of tyrosine 31 (T-P-YP-S-Y).
Buffer
phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clone Name
Isotype
Species Reactivity
Human
Concentration
1ug/ul
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site. (Protein A or G Sepharose)
Guaranteed Application *
WB
Suggested Dilutions
WB: 1:500~1:1000
Background
Related Pathway
EGFR1 Signaling Pathway
Focal Adhesion
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Western blot analysis of extracts from SK-OV3 cells untreated or treated with estradiol (0.1uM, 20min) using Paxillin antibody (Lanes 1 and 2) and Paxillin (phospho-Tyr31) antibody (Lanes 3 and 4).
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