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Anti-PXN PHOSPHO Antibody
|The antiserum was produced against synthesized phosphopeptide derived from humanPaxillin around the phosphorylation site of tyrosine 31 (T-P-YP-S-Y).|
|phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
|Homo sapiens paxillin (PXN), transcript variant 4|
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Western blot analysis of extracts from SK-OV3 cells untreated or treated with estradiol (0.1uM, 20min) using Paxillin antibody (Lanes 1 and 2) and Paxillin (phospho-Tyr31) antibody (Lanes 3 and 4).