Home Antibody All anti-VIM antibodies
|Recombinant human vimentin purified from E. coli|
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1,000, IF: 1:500
|total IgY fraction in PBS + 10 mM NaN3.|
|Total IgY fraction
|Rattus norvegicus vimentin (Vim)|
Entrez Gene 81818 Rat
|This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract. |
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Western blot of NIH 3T3 cells showing specific immunolabeling of the ~50k Vimentin protein.
Mixed neuron/glial cultures stained with anti-Vimentin (green) and rabbit anti-GFAP antibody (red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the image which appear green. Astrocytes may express primarily GFAP, or GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and vimentin, the two proteins assemble to produce heteropolymer filaments.