Home Antibody All anti-UBE2L3 antibodies
Also for UBE2L3 (NM_198157)
|Synthetic peptide corresponding to a region within amino acids 92 and 154 of UBE2L3 (Uniprot ID#P68036)|
|Human (Predicted: Xenopus laevis, Xenopus Tropicalis, Pig, Chicken, Rhesus Monkey, Bovine)
|WB, IHC, IF, IP
||ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
|0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.|
|Purified by antigen-affinity chromatography.
|Seq homology of immunogen across species: Xenopus laevis (100%), Xenopus Tropicalis (100%), Pig (100%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%)
|Homo sapiens ubiquitin-conjugating enzyme E2L 3 (UBE2L3), transcript variant 2|
|E2-F1; L-UBC; UBCH7; UbcM4; L-UBC; UBCH7; UbcM4; ubiquitin carrier protein; ubiquitin-conjugating enzyme UBCH7; ubiquitin-protein ligase; ubiquitin-conjugating enzyme E2L 3|
Entrez Gene 7332 Human
Entrez Gene 699895 Monkey
|The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]|
| Ubiquitin mediated proteolysisParkinson's disease|
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UBE2L3 antibody detects UBE2L3 protein by Western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. 15 % SDS-PAGE. UBE2L3 antibody (TA308758) dilution: 1:1000
Sample (30 ug of whole cell lysate). A: Hela. B: Hep G2 . 12% SDS PAGE. TA308758 diluted at 1:1000.
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using UBE2L3(TA308758) antibody at 1:100 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using UBE2L3(TA308758) antibody at 1:200 dilution.
Immunoprecipitation of UBE2L3 protein from HeLa whole cell extracts using 5 ug of UBE2L3 antibody (TA308758). Western blot analysis was performed using UBE2L3 antibody (TA308758). An anti-rabbit IgG was used as a secondary reagent.