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Anti-ACACA Antibody EPR4971
Also for ACACA (NM_198834)
|A synthetic peptide corresponding to residues in N-terminus of human Acetyl CoA Carboxylase 1 (ACC1) was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||WB: 1:1000 - 1:10000; IHC-P: 1:250 - 1:500; FC: 1:100 - 1:500; ICC/IF: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for IP.
|Homo sapiens acetyl-CoA carboxylase alpha (ACACA), transcript variant 1|
|ACAC; ACACAD; ACC; ACC1; ACCA|
|Acetyl-CoA Carboxylase 1 (ACC1) is a biotin dependent lipogenic enzyme that is highly expressed during adipogenesis. ACC1 catalyzes acetyl-CoA carboxylation, producing malonyl-CoA, a metabolite involved in energy homeostasis regulation. Malonyl-CoA is a two carbon donor in the synthesis of long-chain fatty acids and the elongation of fatty acids found in the cystol (1). ACC1 is regulated short-term by citrate, CoA, and palmitoyl-CoA through allosteric interactions. Nutrients and hormones can be both short-term (inducing reversible phosphorylations by such as AMPK) and long-term (transcription level regulation) regulators of ACC1 (2). Highly expressed in lipogenic tissues, ACC1 is found in liver, adipose, and lactating mammary gland (3). ACC1 has been implicated as a target in the development of anti-obesity drugs (4).|
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Western blot - Acetyl Coenzyme A carboxylase alpha antibody [EPR4971]; All lanes : Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR4971] at 1/1000 dilution.Lane 1 : 293T cell lysate.Lane 2 : HepG2 cell lysate.Lane 3 : SH-SY5Y cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled Goat anti-Rabbit at 1/2000 dilution.Predicted band size : 266 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Acetyl Coenzyme A carboxylase alpha antibody [EPR4971]; Immunohistochemical analysis of paraffin-embedded skeletal muscle tissue using ab109368 at 1/250 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Acetyl Coenzyme A carboxylase alpha antibody [EPR4971]; Immunohistochemical analysis of paraffin-embedded brain tissue using ab109368 at 1/250 dilution.
Immunocytochemistry/ Immunofluorescence - Acetyl Coenzyme A carboxylase alpha antibody [EPR4971]; Immunofluorescent staining of 293 cells using ab109368 at 1/100 dilution
Flow Cytometry - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR4971]; Overlay histogram showing SH-SY5Y cells stained with ab109368 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.