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Home Antibody All anti-CDT1 antibodies

Anti-CDT1 Antibody EPR4729

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307808
  • Rabbit monoclonal antibody against CDT1(clone EPR4729)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC403098) , 20ug
100ul 325 3-7 Days
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WB(1)
FC(1)
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Also for CDT1 (NM_030928)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the C-terminus of human CDT1 was used as an immunogen.
Clone NameEPR4729 IsotypeIgG
Species ReactivityHuman, Mouse, Rat ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, FC Suggested DilutionsWB: 1:1000 - 1:10000; FC: 1:500 - 1:1000
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for ICC,IHC-P or IP.

Reference Data

Target NameHomo sapiens chromatin licensing and DNA replication factor 1 (CDT1)
Alternative NameDUP; RIS2
Database LinkNP_112190
FunctionCDT1 is a novel replication factor conserved throughout evolution, and it was first identified in S. pombe as a target of CDC10 transcriptional regulation. It has been demonstrated that CDT1 cooperates with CDC18/CDC6 in driving re-replication in G2 when the CDC18/CDC6 protein is overexpressed and that CDT1 depletion prevents the binding of MCM proteins to chromatin (1). Regulation of CDT1 plays a critical role in the licensing process. Inhibition of CDT1 activity occurs by multiple mechanisms, including ubiquitin-dependent proteolysis and binding to its potent inhibitor Geminin. Misregulation of the licensing process and defects in the ordered assembly and disassembly of the pre-RC alter the integrity of the genome. Thus, cell cycle regulation of CDT1 expression and function is responsible for licensing replication origins in G1 and preventing relicensing and hence rereplication in S phase.
Related Pathway
Cell cycle

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - CDT1 antibody [EPR4729]; All lanes : Anti-CDT1 antibody [EPR4729] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : 293T cell lysate.Lane 3 : HUVEC cell lysate.Lane 4 : Jurkat cell lysate.Lane 5 : Mouse brain lysate.Lane 6 : Rat brain lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 60 kDa.
FC Image
Flow Cytometry - CDT1 antibody [EPR4729]; Flow cytometric analysis of CDT1 in permeabilized Jurkat cells using TA307808 at 1/500 dilution (red) or a rabbit IgG (negative) (green).

 

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