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Anti-LIPE PHOSPHO Antibody EPR2329(2)
Also for LIPE (NM_005357)
|A phospho-specific peptide corresponding to residues surrounding Serine 853 of human HSL was used as an immunogen. The antibody only detects HSL phosphorylated on Serine 853.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:50000 - 1:200000
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
|Homo sapiens lipase, hormone-sensitive (LIPE)|
|HSL (Hormone-sensitive lipase) is a key enzyme in fatty acid mobilization, overall energy homeostasis, and possibly steroidogenesis. It is acutely controlled through reversible phosphorylation by catecholamines and insulin (1). HSL is the rate-limiting enzyme in hydrolysis of triglycerides in adipose tissue and of cholesteryl esters in steroidogenic tissues including the adrenals, ovaries, testes, and macrophages (2, 3). HSL activity has been positively correlated with free and esterified cholesterol ratios in seminiferous tubules (STf) and interstitial tissue (ITf) enriched fractions, but not with triglyceride levels, during testicular development. HSL is localized to elongated spermatids and Sertoli cells, where its distribution is stage-dependent, and within the cells lining the excurrent ducts of the testis. The HSL protein levels and enzymatic activity in ITf and STf were reported positively correlated with serum testosterone levels during development (4).|
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Western blot - HSL/LIPE (phospho S853) antibody [EPR2329(2)]; All lanes : Anti-Hormone sensitive lipase (phospho S853) antibody [EPR2329(2)] at 1/50000 dilution.Lane 1 : Human skeletal muscle lysates.Lane 2 : Human skeletal muscle lysates treated with Lambda Phosphatase.Lysates/proteins at 10 µg per lane.Predicted band size : 117 kDa.Observed band size : 83 kDa .
Western blot - HSL/LIPE (phospho S853) antibody [EPR2329(2)]; All lanes : Anti-Hormone sensitive lipase (phospho S853) antibody [EPR2329(2)] at 1/50000 dilution.Lane 1 : Mouse muscle lysate.Lane 2 : Mouse muscle lysate treated with Alkaline Phosphatase.Lysates/proteins at 10 µg per lane.Predicted band size : 117 kDa.Observed band size : 83 kDa .