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Anti-CHEK2 Antibody EPR4325
Also for CHEK2 (NM_007194)
|A recombinant protein fragment corresponding to amino acids 43-167 of human Chk2 was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||ICC/IF: Use at an assay dependent concentration; WB: 1:50000 - 1:200000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt.
|Homo sapiens checkpoint kinase 2 (CHEK2), transcript variant 1|
|CDS1; CHK2; hCds1; HuCds1; LFS2; PP1425; RAD53|
|Chk2 is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, Chk2 is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in Chk2 have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this protein are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors (1).|
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Western blot - Chk2 antibody [EPR4325]; All lanes : Anti-Chk2 antibody [EPR4325] at 1/50000 dilution.Lane 1 : HeLa treated with Gamma Irradiation.Lane 2 : HeLa cell lysate.Lane 3 : HT29 cell lysate.Lane 4 : 293T cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 61 kDa.Observed band size : 62 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Chk2 antibody [EPR4325]; Immunohistochemical analysis of paraffin-embedded Human colon tissue using 1/100 ab109413
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Chk2 antibody [EPR4325]; Immunohistochemical analysis of paraffin-embedded Human spleen tissue using 1/100 ab109413
Immunocytochemistry/ Immunofluorescence - Anti-Chk2 antibody [EPR4325]; ab109413 (1/500) staining Chk2 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5%Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please see Abreview.