OriGene Technologies, Inc.
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-CHEK2 antibodies

Anti-CHEK2 Antibody EPR4325

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307792
  • Rabbit monoclonal antibody against Chk2(clone EPR4325)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC416128) , 20ug
100ul $325 3-7 Days
Add to Shopping Cart
WB(1)
IHC(2)
IF(1)
spacer
Also for CHEK2 (NM_007194)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA recombinant protein fragment corresponding to amino acids 43-167 of human Chk2 was used as an immunogen.
Clone NameEPR4325 IsotypeTissue culture supernatant
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF Suggested DilutionsICC/IF: Use at an assay dependent concentration; WB: 1:50000 - 1:200000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250
BufferDoes not react with Mouse, Rat. Is unsuitable for Flow Cyt.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens checkpoint kinase 2 (CHEK2), transcript variant 1
Alternative NameCDS1; CHK2; hCds1; HuCds1; LFS2; PP1425; RAD53
Database LinkNP_009125
FunctionChk2 is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, Chk2 is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in Chk2 have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this protein are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors (1).
Related Pathway
Cell cycle

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Chk2 antibody [EPR4325]; All lanes : Anti-Chk2 antibody [EPR4325] at 1/50000 dilution.Lane 1 : HeLa treated with Gamma Irradiation.Lane 2 : HeLa cell lysate.Lane 3 : HT29 cell lysate.Lane 4 : 293T cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 61 kDa.Observed band size : 62 kDa .
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Chk2 antibody [EPR4325]; Immunohistochemical analysis of paraffin-embedded Human colon tissue using 1/100 TA307792
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Chk2 antibody [EPR4325]; Immunohistochemical analysis of paraffin-embedded Human spleen tissue using 1/100 TA307792
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-Chk2 antibody [EPR4325]; TA307792 (1/500) staining Chk2 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5%Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please see Abreview.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title