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Anti-WFDC2 Antibody EPR4743
Also for WFDC2 (NM_006103)
|A synthetic peptide corresponding to residues in human HE4 was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; ICC: 1:100 - 1:250; FC: 1:100 - 1:500
|Does not react with Rat. Is unsuitable for IHC-P or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for IHC-P or IP.
|Homo sapiens WAP four-disulfide core domain 2 (WFDC2)|
|dJ461P17.6; EDDM4; HE4; WAP5|
|HE4, or WAP four-disulfide core domain 2, is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and it functions as a protease inhibitor in many family members. HE4 is expressed in pulmonary epithelial cells, and it is also found to be expressed in some ovarian cancers. HE4 is a small secretory protein, which may be involved in sperm maturation.|
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Western blot - HE4 antibody [EPR4743]; All lanes : Anti-HE4 antibody [EPR4743] at 1/1000 dilution.Lane 1 : HT29 cell lysate.Lane 2 : SW480 cell lysate.Lane 3 : Ovary cancer tissue lysate.Lane 4 : SK-OV-3 cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 13 kDa.Observed band size : 23 kDa .
Flow Cytometry - HE4 antibody [EPR4743]; ab109298, at 1/100 dilution, staining HE4 in permeabilized SW480 cells (red) and rabbit IgG as a negative control (green) using flow cytometry.