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Anti-IRS2 Antibody EPR904(2)
Also for IRS2 (NM_003749)
|A synthetic peptide corresponding to residues in human IRS-2 was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:1000 - 1:10000; IHC-P: 1:50 - 1:100; FC: 1:100 - 1:1000
|Does not react with Rat. Is unsuitable for ICC or IP.|
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA (Protein A or G Sepharose)
|Is unsuitable for ICC or IP.
|Homo sapiens insulin receptor substrate 2 (IRS2)|
|Insulin receptor substrates (IRS), the major intracellular substrates of the insulin receptor (IR), are adaptor proteins that transduce signals from the IR to downstream effectors that are important for the biological effect of insulin (1-2). After insulin stimulation, IRS proteins are rapidly phosphorylated on multiple tyrosine residues. Once phosphorylated, IRS proteins bind and activate Grb-2, SHP2 and the PI3-K p85 subunit (2-3). Sequences of IRS-2 and IRS-1 reveal a highly conserved amino terminus containing a pleckstrin-homology domain and a phosphotyrosine-binding domain, and a poorly conserved carboxy terminus containing several tyrosine phosphorylation motifs. IRS-2 is expressed in many cells, including tissues from IRS-1-/- mice, and may be essential for signaling by several receptor systems such as insulin and cytokine (1).|
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Western blot - Anti-IRS2 antibody [EPR904(2)]; All lanes : Anti-IRS2 antibody [EPR904(2)] at 1/1000 dilution.Lane 1 : 293T cell lysate.Lane 2 : 293T + insulin cell lysate.Lane 3 : SH-SY5Y cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 137 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRS2 antibody [EPR904(2)]; Immunohistochemical analysis of paraffin-embedded Human breast tissue labelling IRS2 with ab134101 at 1/50 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRS2 antibody [EPR904(2)]; Immunohistochemical analysis of paraffin-embedded Human muscle tissue labelling IRS2 with ab134101 at 1/50 dilution.
Flow Cytometry - Anti-IRS2 antibody [EPR904(2)]; Overlay histogram showing Hela cells stained with ab134101 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.