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Anti-GSN Antibody EPR1942

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307746
  • Rabbit monoclonal antibody against Gelsolin(clone EPR1942)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC424875) , 20ug
100ul 325 In Stock
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WB(1)
IHC(2)
IF(1)
FC(1)
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Also for GSN (NM_000177)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human Gelsolin was used as an immunogen.
Clone NameEPR1942 IsotypeTissue culture supernatant
Species ReactivityHuman, Mouse ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:50000 - 1:20000; IHC-P: 1:250 - 1:500; FC: 1:50 - 1:1000; ICC/IF: 1:100 - 1:250
BufferDoes not react with Rat. Is unsuitable for IP.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens gelsolin (GSN), transcript variant 1
Alternative NameADF; AGEL
Database LinkNP_000168
FunctionGelsolin is a calcium regulated actin binding protein. Gelsolin is a regulator of actin filament assembly and disassembly and consists of six homologous subdomains (S1-S6). When C-terminal tail (S6) is bound to calcium ion, it straightens and exposes actin binding sites located on S2s helices, leading to Gelsolin actin-serving activity (1). Gelsolin is inhibited by phosphatidylinositol (4,5)-bisphosphate (PIP2), where PIP2 will bind to S1, S2, and S3 sites. Gelsolin is involved in podosome formation and has been linked to inhibition of apoptosis by preventing release of cytochrome C (2).
Related Pathway
Senescence and Autophagy

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Gelsolin antibody [EPR1942]; All lanes : Anti-Gelsolin antibody [EPR1942] at 1/50000 dilution.Lane 1 : MCF7 cell lysate.Lane 2 : HL60 cell lysate.Lane 3 : A431 cell lysate.Lane 4 : HeLa cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 86 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Gelsolin antibody [EPR1942]; Immunohistochemical analysis of Gelsolin expression in paraffin-embedded Human kidney tissue using TA307746 at 1/250 dilution.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Gelsolin antibody [EPR1942]; Immunohistochemical analysis of Gelsolin expression in paraffin-embedded Human lung tissue using TA307746 at 1/250 dilution.
IF Image
Immunocytochemistry/ Immunofluorescence - Gelsolin antibody [EPR1942]; Immunofluorescent staining of Gelsolin in HeLa cells using TA307746 at 1/100 dilution.
FC Image
Flow Cytometry - Anti-Gelsolin antibody [EPR1942]; Overlay histogram showing HL60 cells stained with TA307746 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HL60 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

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